2024: Volume 4, Issue 1
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PDFDevelopment of Nanoparticles Containing Bromealin
Íngara São Paulo1, Sandra A de Assis1, Fernanda Vidigal Souza2, Elaine M. Cabral-Albuquerque3, Cassia Britto Detoni4,*
1Enzymology and Fermentation Technology Laboratory, Health Department, State University of Feira de Santana, Feira de Santana, Brazil
2EMBRAPA Centro Nacional de Pesquisa de Mandioca e Fruticultura Tropical, Brazil
3Departamento de Engenharia Química, Escola Politecnica, Federal University of Bahia, Brazil
4Instituto de Ciências Farmacêuticas – Centro Multidisciplinar de Macaé, Federal University of Rio de Janeiro. Av. Aluizio da Silva Gomes, 50 - Novo Cavaleiros, Macaé - RJ, Brasil
*Corresponding Author: Cassia Britto Detoni, Instituto de Ciências Farmacêuticas – Centro Multidisciplinar de Macaé, Federal University of Rio de Janeiro. Av. Aluizio da Silva Gomes, 50 - Novo Cavaleiros, Macaé - RJ, Brasil, CEP 27930-560; Email: [email protected]
Received Date: January 23, 2024
Publication Date: March 20, 2024
Citation: Paulo IS, et al. (2024). Development of Nanoparticles Containing Bromealin. Dermis. 4(1):7.
Copyright: Paulo IS, et al. © (2024).
ABSTRACT
Bromelain is a protein complex containing proteases and other non-proteolytic constituents, with several therapeutic applications, such as: debriding, antineoplastic, anticoagulant and anti-inflammatory. The present work aimed to produce Eudragit® S100 nanoparticles (NP) containing bromelain extracted from a hybrid pineapple, Ananas comosus var. Erectifolius x Ananas comosus var. Bracteatus, characterize the nanocarrier and study optimal enzyme pH and temperature. NP were prepared by nanoprecipitation, using 2 polymer concentrations (1 and 2 mg/mL). Nanoparticles with adequate size (D[4,3] = 386 nm) and size distribution (Span= 0.6) were obtained when a lower polymer concentration was used. After adsorption, an increase in particle size (486 nm) and change the zeta potential (from -19 mV to -5.5 mV) was verified. Total protein quantification after immobilization showed a small loss. The association efficiency (AE%) of total protein was 45.24%, and the catalytic activity was maintained after the adsorption. A 3 Level Factorial Design was used to study the optimal pH (7.15) and temperature (52.5 °C) for free enzyme, and optimal pH (6.77) and temperature (52.5 °C) for adsorbed bromelain. This work revealed the viability of bromelain immobilization by adsorption to Eudragit S100 nanoparticles, which had a protective effect on the enzymatic activity during storage.
